Equipment
Listed below are the major microscopes, ancillary equipment, and facilities available to users of the Microscopy Suite. Click the equipment name for more information on each piece of equipment.
Please be aware that the ITG staff cannot respond to questions about particular pieces of equipment or software packages unless those questions are related to research conducted in the ITG laboratories. The ITG does not sell, lease, or distribute equipment or software, save that clearly marked as developed by the ITG.
Equipment
Widefield FRET & TIRF Microscope
The Zeiss Axiovert 200M is configured to be capable of widefield Fluorescence Resonance Energy Transfer (FRET) microscopy and Total Internal Reflection Fluorescence (TIRF) microscopy. The microscope is equipped with a cooled Photometrics 512 Evolve EMCCD camera with high sensitivity for quantitative low-light fluorescence imaging.
FRET microscopy allows the determination of the approach between two molecules within several nanometers, a distance sufficiently close for molecular interactions to occur. For the FRET microscopy, the imaging and system control is realized by using the software MetaFluor. The filter sets installed in the microscope allow imaging a number of FRET pairs, such as ECFP-YPet.
TIRF microscopy can be used to observe the fluorescence of a single molecule or a thin region of a specimen, usually less than 200 nanometers thick. Currently three lasers (the wavelengths are 405 nm, 473 nm and 561 nm) are available for the TIRF imaging.
User Resources
For additional information about this piece of equipment, see the Calendars, Contacts, and Fees pages.
| Primary Contacts | |
|---|---|
| Secondary Contacts | |
| Manufacturer | Carl Zeiss, Inc. |
| Equipment Model | Axiovert 200M |
| Location | B0420A |
| Phone Numbers | (217) 333-4387, (217) 265-5071 |
Facilities
Wet Lab - B612
Bench space, fume hood, some chemicals and solvents, dual source metal evaporator, pH meter, dissecting scope, microbalance, glow discharge system, reverse osmosis water purification system, sonicator, sputter coater, critical point dryer, low speed bone saw, osmium plasma coater, and other equipment.
BL-2 Lab - B420
Bench space, biosafety cabinet for sample manipulations, centrifuge, and two incubators for short-term sample storage.
Microscopy Suite
405 North Mathews Avenue, Urbana, IL
61801 USA
(217) 300-0566