Equipment
Listed below are the major microscopes, ancillary equipment, and facilities available to users of the Microscopy Suite. Click the equipment name for more information on each piece of equipment.
Please be aware that the ITG staff cannot respond to questions about particular pieces of equipment or software packages unless those questions are related to research conducted in the ITG laboratories. The ITG does not sell, lease, or distribute equipment or software, save that clearly marked as developed by the ITG.
Equipment
Leica SP8 UV/Visible Laser Confocal Microscope
Confocal microscopy permits one to optically section a fluorescent sample (such as a cell that has been stained with contrasting fluorescent dyes) with superior resolution by using a pinhole to reject light that originates outside of the chosen area. By collecting a series of such images through the depth of a sample, the user may assemble a highly accurate three-dimensional reconstruction of the entire sample.
Features:
- Laser lines: 405, 458, 488, 514, 561 & 633 nm.
- Four detectors: Three PMTs + one high-sensitivity GaAsP HyD detectors.
- Resonant scanning (8kHz) allows 28 fps at 512*512 for fast live cell imaging.
- Scan optics for high transmission from 400 nm to 1300 nm.
- Optical scanfield rotation up to 200 degrees.
- Three mirrors X2Y scanner for parallax-free and tiling imaging.
- Motorized stage, mark & find, tiling confocal imaging.
- LAS X control software.
Objectives:
- 20x/0.75 IMM (oil, water, glycerol) CORR CS2, free Working distance 670 um
- 40x/1.30 HC PL APO Oil CS2, free Working distance 240 um
- 63x/1.40 HC PL APO Oil CS2, free working distance 140 um
Application Modules:
- Fluorescence Recovery After Photobleaching (FRAP).
- Fluorescence Resonance Energy Transfer (FRET)
User Resources
For additional information about this piece of equipment, see the Calendars, Contacts, and Fees pages.
| Primary Contacts | |
|---|---|
| Secondary Contacts | |
| Manufacturer | Leica Microsystems |
| Equipment Model | TCS SP8 |
| Location | B0420B |
| Phone Numbers | (217) 333-4387, (217) 265-5071, (217) 244-6270 (Room B420B) |
Facilities
Wet Lab - B612
Bench space, fume hood, some chemicals and solvents, dual source metal evaporator, pH meter, dissecting scope, microbalance, glow discharge system, reverse osmosis water purification system, sonicator, sputter coater, critical point dryer, low speed bone saw, osmium plasma coater, and other equipment.
BL-2 Lab - B420
Bench space, biosafety cabinet for sample manipulations, centrifuge, and two incubators for short-term sample storage.
Microscopy Suite
405 North Mathews Avenue, Urbana, IL
61801 USA
(217) 300-0566